Secondary-Ion Mass Spectrometry of Genetically Encoded Targets**

نویسندگان

  • Ingrid C Vreja
  • Selda Kabatas
  • Sinem K Saka
  • Katharina Kröhnert
  • Carmen Höschen
  • Felipe Opazo
  • Ulf Diederichsen
  • Silvio O Rizzoli
چکیده

Secondary ion mass spectrometry (SIMS) is generally used in imaging the isotopic composition of various materials. It is becoming increasingly popular in biology, especially for investigations of cellular metabolism. However, individual proteins are difficult to identify in SIMS, which limits the ability of this technology to study individual compartments or protein complexes. We present a method for specific protein isotopic and fluorescence labeling (SPILL), based on a novel click reaction with isotopic probes. Using this method, we added (19) F-enriched labels to different proteins, and visualized them by NanoSIMS and fluorescence microscopy. The (19) F signal allowed the precise visualization of the protein of interest, with minimal background, and enabled correlative studies of protein distribution and cellular metabolism or composition. SPILL can be applied to biological systems suitable for click chemistry, which include most cell-culture systems, as well as small model organisms.

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عنوان ژورنال:

دوره 54  شماره 

صفحات  -

تاریخ انتشار 2015